Difference between revisions of "IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/09"

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For Lucy's experiments, a colony from each of the four transformation plates was inoculated into LB (2 samples with Kan. and Cm, 2 samples with Kan. only) and left to grow overnight at 37°C in a shaking incubator.  
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For the protein experiments, a colony from each of the four transformation plates was inoculated into LB (2 samples with Kanamycin and Chlorophenicol, 2 samples with Kanamycin only) and left to grow overnight at 37°C in a shaking incubator.  
  
[[Image:9-7-2013 (2).jpg |right|300px]]
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[[Image:9 july 2.jpg|thumb|Fig. PCR results run on agarose gel electrophoresis, where sample 1 is the control with no DNA added, sample 2 is the AntGp-Neo-Xba1 biobrick and sample 3 is the J04450-Nde1 biobrick]]
  
  

Revision as of 09:27, 5 August 2013

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PCR samples were retrieved and run on a gel, This was followed by the transformation of samples into bacterial cells, which was performed following the kit's protocol. Samples were plated on agar plates and incubated overnight at 37°C


For the protein experiments, a colony from each of the four transformation plates was inoculated into LB (2 samples with Kanamycin and Chlorophenicol, 2 samples with Kanamycin only) and left to grow overnight at 37°C in a shaking incubator.

Fig. PCR results run on agarose gel electrophoresis, where sample 1 is the control with no DNA added, sample 2 is the AntGp-Neo-Xba1 biobrick and sample 3 is the J04450-Nde1 biobrick