Difference between revisions of "IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/09"

From OpenWetWare
Jump to: navigation, search
Line 12: Line 12:
 
For Lucy's experiments, a colony from each of the four transformation plates was inoculated into LB (2 samples with Kanamycin and Chlorophenicol, 2 samples with Kanamycin only) and left to grow overnight at 37°C in a shaking incubator.  
 
For Lucy's experiments, a colony from each of the four transformation plates was inoculated into LB (2 samples with Kanamycin and Chlorophenicol, 2 samples with Kanamycin only) and left to grow overnight at 37°C in a shaking incubator.  
  
[[Image:9-7-2013 (2).jpg |right|100px]]
+
[[Image:156px-9-7-2013 (2).jpg|thumb|Fig. PCR results run on agarose gel electrophoresis]]
  
  

Revision as of 04:13, 31 July 2013

Igem-logo-150px.png Week Six <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

PCR samples were retrieved and run on a gel, This was followed by the transformation of samples into bacterial cells, which was performed following the kit's protocol. Samples were plated on agar plates and incubated overnight at 37°C


For Lucy's experiments, a colony from each of the four transformation plates was inoculated into LB (2 samples with Kanamycin and Chlorophenicol, 2 samples with Kanamycin only) and left to grow overnight at 37°C in a shaking incubator.

Fig. PCR results run on agarose gel electrophoresis