Difference between revisions of "IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/09"

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Six</span>
 
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Six</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Floor Two==
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PCR samples were retrieved and run on a gel ''fig 1'', This was followed by the transformation of samples into bacterial cells, which was performed following the kit's protocol. Samples were plated on agar plates and incubated overnight at 37°C
  
PCR samples were retrieved and run on a gel, This was followed by the transformation of samples into bacterial cells, which was performed following the kit's protocol. Samples were plated on agar plates and incubated overnight at 37°C
 
  
  
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For the protein experiments, a colony from each of the four transformation plates was inoculated into LB (2 samples with Kanamycin and Chlorophenicol, 2 samples with Kanamycin only) and left to grow overnight at 37°C in a shaking incubator.
  
For Lucy's experiments, a colony from each of the four transformation plates was inoculated into LB (2 samples with Kan. and Cm, 2 samples with Kan. only) and left to grow overnight at 37°C in a shaking incubator.
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[[Image:9 july 2.jpg|thumb|Fig 1: PCR results run on agarose gel electrophoresis, where sample 1 is the control with no DNA added, sample 2 is the AntGp-Neo-Xba1 biobrick and sample 3 is the J04450-Nde1 biobrick]]
 
 
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Latest revision as of 22:03, 26 September 2017

Igem-logo-150px.png Week Six Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Floor Two

PCR samples were retrieved and run on a gel fig 1, This was followed by the transformation of samples into bacterial cells, which was performed following the kit's protocol. Samples were plated on agar plates and incubated overnight at 37°C


For the protein experiments, a colony from each of the four transformation plates was inoculated into LB (2 samples with Kanamycin and Chlorophenicol, 2 samples with Kanamycin only) and left to grow overnight at 37°C in a shaking incubator.

Fig 1: PCR results run on agarose gel electrophoresis, where sample 1 is the control with no DNA added, sample 2 is the AntGp-Neo-Xba1 biobrick and sample 3 is the J04450-Nde1 biobrick