IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/06/26
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Cultures grown overnight were retrieved and DNA mini preps was performed. This was followed by restriction digest reactions using EcoR1 and Pst1 enzymes. Samples were then run on an agarose gel, fig 1, to confirm the digest reactions.