Difference between revisions of "IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/06/26"

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Cultures grown overnight were retrieved and DNA mini preps was performed. This was followed by restriction digest reactions using EcoR1 and Pst1 enzymes. Samples were then run on an agarose gel to confirm the digest reactions.
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Cultures grown overnight were retrieved and DNA mini preps was performed. This was followed by restriction digest reactions using EcoR1 and Pst1 enzymes. Samples were then run on an agarose gel, ''fig 1,'' to confirm the digest reactions.
  
[[Image:26-6-2013.jpg |right|300px]]
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[[Image:800px-26 june.jpg|right|thumb|Fig 1:Analysis of restriction digest products of ligated, transformed plasmids using agrose gel electrophoresis. Samples shown are the control (original J04450 plasmid), and colonies 1-6 respectively)
 
 
Also, it was today in which we discovered and confirmed that our AntgP Neo sequence was wrong, missing the Xba1 restriction site. So we had to do further planning for potential experiments.
 
  
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.]].Also, it was today in which we discovered and confirmed that our AntgP Neo sequence was wrong, missing the Xba1 restriction site. So we had to do further planning for potential experiments.
  
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Revision as of 04:12, 9 August 2013

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Cultures grown overnight were retrieved and DNA mini preps was performed. This was followed by restriction digest reactions using EcoR1 and Pst1 enzymes. Samples were then run on an agarose gel, fig 1, to confirm the digest reactions.

Fig 1:Analysis of restriction digest products of ligated, transformed plasmids using agrose gel electrophoresis. Samples shown are the control (original J04450 plasmid), and colonies 1-6 respectively) .
.Also, it was today in which we discovered and confirmed that our AntgP Neo sequence was wrong, missing the Xba1 restriction site. So we had to do further planning for potential experiments.