IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/08/05: Difference between revisions
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(Autocreate 2014/08/05 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM) |
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== | ==Week 8, Day 2== | ||
Lab work | |||
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- Added 2g of sucrose and 0.441g of MS salts to 100ml of sterile water. Added Acetosyringone. | |||
- Spun down agrobacterium cultures grown on 4.8.14 | |||
- Resuspended pellets in 10ml of prepared solution | |||
- Left to grow on the bench for 3 hours | |||
- Each construct infiltrated into one nicotiana benthamiana plant on two separate leaves | |||
- Mini preps of level 1 dig-ligs (1,5,9,15,16,17,18,19) | |||
- Quantification of DNA required for next level two dig-ligs | |||
Revision as of 08:54, 6 August 2014
 iGEM Project name 1
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Week 8, Day 2Lab work - Added 2g of sucrose and 0.441g of MS salts to 100ml of sterile water. Added Acetosyringone. - Spun down agrobacterium cultures grown on 4.8.14 - Resuspended pellets in 10ml of prepared solution - Left to grow on the bench for 3 hours - Each construct infiltrated into one nicotiana benthamiana plant on two separate leaves
- Quantification of DNA required for next level two dig-ligs
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