IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/08/05: Difference between revisions

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Entry title==
==Week 8, Day 2==
* Insert your content here.


Lab work
----
- Added 2g of sucrose and 0.441g of MS salts to 100ml of sterile water.  Added Acetosyringone.
- Spun down agrobacterium cultures grown on 4.8.14
- Resuspended pellets in 10ml of prepared solution
- Left to grow on the bench for 3 hours
- Each construct infiltrated into one nicotiana benthamiana plant on two separate leaves
- Mini preps of level 1 dig-ligs (1,5,9,15,16,17,18,19)
- Quantification of DNA required for next level two dig-ligs




Latest revision as of 00:10, 27 September 2017

iGEM Project name 1 Main project page
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Week 8, Day 2

Lab work

- Added 2g of sucrose and 0.441g of MS salts to 100ml of sterile water. Added Acetosyringone.

- Spun down agrobacterium cultures grown on 4.8.14

- Resuspended pellets in 10ml of prepared solution

- Left to grow on the bench for 3 hours

- Each construct infiltrated into one nicotiana benthamiana plant on two separate leaves


- Mini preps of level 1 dig-ligs (1,5,9,15,16,17,18,19)

- Quantification of DNA required for next level two dig-ligs



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