IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/02: Difference between revisions
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==Floor One== | ==Floor One== | ||
[[Image:GEL3.png|thumb| Fig 1. Gel showing another GUS PCR, with a 1Kb+ base ladder in lane 2 and a band of GUS product at roughly 1.8Kb in lane 3.]] | |||
The PCR of the new GUS gene was performed (8 x 20 ul reactions of same proportions as first time round).<br> | The PCR of the new GUS gene was performed (8 x 20 ul reactions of same proportions as first time round).<br> | ||
A 1% agarose gel was made.<br> | A 1% agarose gel was made.<br> | ||
Took photographs of all Bioassay plates where actinomycete colonies appeared to have a zone of clearing around them - indicating the production of an anti-fungal.<br> | Took photographs of all Bioassay plates where actinomycete colonies appeared to have a zone of clearing around them - indicating the production of an anti-fungal.<br> | ||
Spore stocks were filtered, concentrated and resuspended in 20 % glycerol.<br> | Spore stocks were filtered, concentrated and resuspended in 20 % glycerol.<br> |
Revision as of 09:44, 29 September 2013
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Floor OneThe PCR of the new GUS gene was performed (8 x 20 ul reactions of same proportions as first time round). Floor TwoThe plates that had been transformed with the ligation (J04450+neo) contained no colonies.
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