IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/20: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Lucy Clark (talk | contribs) No edit summary |
|||
| Line 5: | Line 5: | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
==Floor One== | |||
Made 6 x 500 ml SFM agar media, autoclaved for 1 hr and poured into plates. | |||
Isolated more single colonies that resembled Streptomyces from soil sample plates. | |||
==Floor Two== | ==Floor Two== | ||
We inoculated cultures of BL21 containing pETAntA and Antgp+RFP into 10mls LB with antibiotics Kanamycin and Chlorophenicol. Four cultures were grown overnight at 37°C. | We inoculated cultures of BL21 containing pETAntA and Antgp+RFP into 10mls LB with antibiotics Kanamycin and Chlorophenicol. Four cultures were grown overnight at 37°C. | ||
Revision as of 08:02, 22 August 2013
 Week Twelve
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Floor OneMade 6 x 500 ml SFM agar media, autoclaved for 1 hr and poured into plates. Isolated more single colonies that resembled Streptomyces from soil sample plates.
Floor TwoWe inoculated cultures of BL21 containing pETAntA and Antgp+RFP into 10mls LB with antibiotics Kanamycin and Chlorophenicol. Four cultures were grown overnight at 37°C.
| |
