IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/11: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 7: Line 7:
==Lab==
==Lab==


Cultures were retrieved from incubation and minipreps were performed following the protocol. This was followed by a restriction enzyme digest reactions using Xba1 and Nde1 enzymes each relevant to its DNA .After incubation samples were run on a gel to confirm the results.
Cultures were retrieved from incubation and minipreps were performed following the protocol ''fig 2''. This was followed by a restriction enzyme digest reactions using Xba1 and Nde1 enzymes each relevant to its DNA .After incubation samples were run on a gel ''fig 3''to confirm the results.


[[Image:2013-07-11 cut sites confirmation.jpg|thumb|Fig. Analysis of restriction digest. Antgp plasmid cut with Xba1 and J04450 plasmid cut with Nde1 in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain digested samples 2a,2b,2c, with Xba1 3a,3b,3c  with Nde1 respectively.]]  
[[Image:2013-07-11 cut sites confirmation.jpg|thumb|Fig 3: Analysis of restriction digest. Antgp plasmid cut with Xba1 and J04450 plasmid cut with Nde1 in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain digested samples 2a,2b,2c, with Xba1 3a,3b,3c  with Nde1 respectively.]]  


[[Image:FMHw4NaVv1qk6t2gg07NMBDmEMwdn6mFeHg2ZQIP6NU.jpg|thumb|Cultures grown overnight after inoculation of 3 colonies from the J04450-Nde1 plate (3a,b,c), showing a pink colour with slightly different intensities. ]]
[[Image:FMHw4NaVv1qk6t2gg07NMBDmEMwdn6mFeHg2ZQIP6NU.jpg|thumb|Fig 2:Cultures grown overnight after inoculation of 3 colonies from the J04450-Nde1 plate (3a,b,c), showing a pink colour with slightly different intensities. ]]




Revision as of 11:28, 22 August 2013

Week Six <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Lab

Cultures were retrieved from incubation and minipreps were performed following the protocol fig 2. This was followed by a restriction enzyme digest reactions using Xba1 and Nde1 enzymes each relevant to its DNA .After incubation samples were run on a gel fig 3to confirm the results.

Fig 3: Analysis of restriction digest. Antgp plasmid cut with Xba1 and J04450 plasmid cut with Nde1 in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain digested samples 2a,2b,2c, with Xba1 3a,3b,3c with Nde1 respectively.
Fig 2:Cultures grown overnight after inoculation of 3 colonies from the J04450-Nde1 plate (3a,b,c), showing a pink colour with slightly different intensities.


The four cultures for the protein expression were recovered and centrifuged to provide a pellet, yielding eight pellets, two from each culture.

Outreach

As the UK meet up was the following day, we practiced and finalised our presentation.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM/2013/07/11&oldid=718669"