IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/12: Difference between revisions
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2. DNA electrophoresis and isolation of DNA encoding AID from proper band. | 2. DNA electrophoresis and isolation of DNA encoding AID from proper band. | ||
3. Digestion of pMPM-T5 with HindIII and NcoI, use of | 3. Digestion of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP). | ||
4. DNA electrophoresis and isolation of pMPM-T5 backbone from proper band. | 4. DNA electrophoresis and isolation of pMPM-T5 backbone from proper band. | ||
Revision as of 01:19, 12 June 2008
 University of Warsaw iGEM Team 2008
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1. Digestion of pTrc99A-AID with HindIII and NcoI. 2. DNA electrophoresis and isolation of DNA encoding AID from proper band. 3. Digestion of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP). 4. DNA electrophoresis and isolation of pMPM-T5 backbone from proper band. 5. DNA ligation (AID + pMPM-T5 backbone) | |
