Difference between revisions of "IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/12"

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2. DNA electrophoresis and isolation of DNA encoding AID from proper band.
 
2. DNA electrophoresis and isolation of DNA encoding AID from proper band.
  
3. Digestion of pMPM-T5 with HindIII and NcoI, use of alcalic phosphatase (CIAP).
+
3. Digestion of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP).
  
 
4. DNA electrophoresis and isolation of pMPM-T5 backbone from proper band.
 
4. DNA electrophoresis and isolation of pMPM-T5 backbone from proper band.

Revision as of 00:19, 12 June 2008

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1. Digestion of pTrc99A-AID with HindIII and NcoI.

2. DNA electrophoresis and isolation of DNA encoding AID from proper band.

3. Digestion of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP).

4. DNA electrophoresis and isolation of pMPM-T5 backbone from proper band.

5. DNA ligation (AID + pMPM-T5 backbone)