IGEM:UNAM-Genomics Mexico/2009/Notebook/Collaborations/2010/10/05: Difference between revisions

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==Entry title==
==Resending dried DNA from Edinburgh?==
* Insert your content here.
*Edinburgh is surprised that the parts they sent us on Sept 18 haven't arrived. They offer sending it again, but also including a LovTAP+RFP reporter construct.
*Luis Told Edinburgh that the best way to send it now is to dry the plasmids in a filter and send them as documents.
*Chris will check out what he needs to dry out the DNA in plasmids.
*Luis assumes Genomics accept receiving LovTAP+RFP
 
===Mail from Chris French, october 5===
 
Dear Luis,
 
sounds like a good plan. I'll look into what is involved in drying DNA 
and get back to you tomorrow or so. We also have a new part you might 
be interested in: we've tried to make a single construct in pSB1C3 
with the Lov-Tap gene (yours) plus the reporter with RFP from 
Lausanne. Should get the sequence back tomorrow, so if it has worked, 
and if this would be useful to you, I can put this in as well.
 
cheers,
 
Chris F.
 
 
On 5 Oct 2010, at 17:24, Luis Fernando Montaño Gutiérrez wrote:
===Mail from Luis that Chris answered above===
> Dear Chris,
>
> Actually it's kind of expected that it hasn't arrived. thy told me 
> they
> waited almost a month last time, perhaps a little bit less.
> Of course it would help a lot to send it again through a couriering
> company, but, as you said the problem is customs-related. If the 
> package
> is declared as E coli plasmids, DNA or bacteria, the permits will also
> last a month, so it would be practically the same. However, if the
> shipment is declared as synthetic oligos, the paperwork will be 
> ready in a
> week, more or less, and then you could freely send it to the airport
> address through DHL or UPS, but not FedEx as far as i know. What 
> happened
> some time ago is that you'd rather declare the shipment as what it 
> was,
> and we assumed it would be the same this time (though synthetic 
> oligos is
> not that far from reality... :D)
>
> So, if it's not a problem for you to send it again (and therefore 
> declare
> it as synthetic oligos), please let us submit the form to customs
> meanwhile we wait for the previous package. In a week we will 
> finally know
> if it is worth sending given that the other one hasn't arrived, so 
> for the
> moment, what do you think of the declaration thing? and by the way, 
> how
> many eppendorf tubes are we expecting?
>
> Oh! I just remembered another option! it happens that if we receive te
> package as a document, there is absolutely no issue with customs, 
> not even
> the need to declare or submit permits. This would mean drying out each
> plasmid solution separately on filter paper. that way it can be sent
> through DHL. But we have never done that before, so we don't know how
> complicated it can be to actually do that, or if you need any special
> equipment to do it. Maybe you do know! that could even be the best 
> option,
> and you can send it to Enrique Paz to the address we have given you
> before.
>
> What do you think?  thanks for all the time spent on these things.
>
> Cheers,
>
> Luis
>




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Resending dried DNA from Edinburgh?

  • Edinburgh is surprised that the parts they sent us on Sept 18 haven't arrived. They offer sending it again, but also including a LovTAP+RFP reporter construct.
  • Luis Told Edinburgh that the best way to send it now is to dry the plasmids in a filter and send them as documents.
  • Chris will check out what he needs to dry out the DNA in plasmids.
  • Luis assumes Genomics accept receiving LovTAP+RFP

Mail from Chris French, october 5

Dear Luis,

sounds like a good plan. I'll look into what is involved in drying DNA and get back to you tomorrow or so. We also have a new part you might be interested in: we've tried to make a single construct in pSB1C3 with the Lov-Tap gene (yours) plus the reporter with RFP from Lausanne. Should get the sequence back tomorrow, so if it has worked, and if this would be useful to you, I can put this in as well.

cheers,

Chris F.


On 5 Oct 2010, at 17:24, Luis Fernando Montaño Gutiérrez wrote:

Mail from Luis that Chris answered above

> Dear Chris, > > Actually it's kind of expected that it hasn't arrived. thy told me > they > waited almost a month last time, perhaps a little bit less. > Of course it would help a lot to send it again through a couriering > company, but, as you said the problem is customs-related. If the > package > is declared as E coli plasmids, DNA or bacteria, the permits will also > last a month, so it would be practically the same. However, if the > shipment is declared as synthetic oligos, the paperwork will be > ready in a > week, more or less, and then you could freely send it to the airport > address through DHL or UPS, but not FedEx as far as i know. What > happened > some time ago is that you'd rather declare the shipment as what it > was, > and we assumed it would be the same this time (though synthetic > oligos is > not that far from reality... :D) > > So, if it's not a problem for you to send it again (and therefore > declare > it as synthetic oligos), please let us submit the form to customs > meanwhile we wait for the previous package. In a week we will > finally know > if it is worth sending given that the other one hasn't arrived, so > for the > moment, what do you think of the declaration thing? and by the way, > how > many eppendorf tubes are we expecting? > > Oh! I just remembered another option! it happens that if we receive te > package as a document, there is absolutely no issue with customs, > not even > the need to declare or submit permits. This would mean drying out each > plasmid solution separately on filter paper. that way it can be sent > through DHL. But we have never done that before, so we don't know how > complicated it can be to actually do that, or if you need any special > equipment to do it. Maybe you do know! that could even be the best > option, > and you can send it to Enrique Paz to the address we have given you > before. > > What do you think? thanks for all the time spent on these things. > > Cheers, > > Luis >



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