1. Volumes to add:
*5 uL of plasmid *3.2 uL of 1 uM sequencing primer *4.8 uL of water total= 13 uL 2. Label constructs iG1,iG2, etc.
3. Record actual plasmid name correlating to iG1, iG2, etc.
4. Print 2 copies (one for the sequencing people and one for Rumi)