Difference between revisions of "IGEM:UC Berkeley/2006/Sequencing"

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  1. Volumes to add:
+
  1. Volumes to add:<br>
 
   *5 uL of plasmid
 
   *5 uL of plasmid
 
   *3.2 uL of 1 uM sequencing primer
 
   *3.2 uL of 1 uM sequencing primer
 
   *4.8 uL of water
 
   *4.8 uL of water
   total= 13 uL
+
   total= 13 uL<br>
  2. Print 2 copies
+
  2. Label constructs iG1,iG2, etc.<br>
 +
3. Record actual plasmid name correlating to iG1, iG2, etc.<br>
 +
4. Print 2 copies (one for the sequencing people and one for Rumi)<br>
 +
 
 +
-JL_06/14/06

Latest revision as of 10:51, 14 June 2006

1. Volumes to add:
*5 uL of plasmid *3.2 uL of 1 uM sequencing primer *4.8 uL of water total= 13 uL
2. Label constructs iG1,iG2, etc.
3. Record actual plasmid name correlating to iG1, iG2, etc.
4. Print 2 copies (one for the sequencing people and one for Rumi)

-JL_06/14/06