IGEM:UC Berkeley/2006/Plasmid Transformation

KCM-competent cell heat shock

1. create cocktail on ice

• 20 uL TGI cells
• 10 uL KCM buffer
• 70 uL water
• 1 uL plasmid

1. heat shock for 1.5 min at 42 degrees C
2. incubate on ice for 5 min.
3. plate