Difference between revisions of "IGEM:UC Berkeley/2006/Plasmid Transformation"

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'''KCM-competent cell heat shock'''<br>
 
'''KCM-competent cell heat shock'''<br>
 
1. create cocktail on ice<br>
 
1. create cocktail on ice<br>
  20 uL TGI cells<br>
+
  *20 uL TGI cells<br>
  10 uL KCM buffer<br>
+
  *10 uL KCM buffer<br>
  70 uL water<br>
+
  *70 uL water<br>
  1 uL plasmid<br>
+
  *1 uL plasmid<br>
 
2. heat shock for 1.5 min at 42 C <br>
 
2. heat shock for 1.5 min at 42 C <br>
 
3. incubate on ice for 5 min. <br>
 
3. incubate on ice for 5 min. <br>
 
4. plate <br>
 
4. plate <br>
 
-JL_06/09/06
 
-JL_06/09/06

Revision as of 13:06, 9 June 2006

KCM-competent cell heat shock
1. create cocktail on ice

  *20 uL TGI cells
*10 uL KCM buffer
*70 uL water
*1 uL plasmid

2. heat shock for 1.5 min at 42 C
3. incubate on ice for 5 min.
4. plate
-JL_06/09/06