Difference between revisions of "IGEM:UC Berkeley/2006/Plasmid Transformation"

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'''KCM-competent cell heat shock'''<br>
 
'''KCM-competent cell heat shock'''<br>
#create cocktail on ice
+
1. create cocktail on ice<br>
 
   20 uL TGI cells<br>
 
   20 uL TGI cells<br>
 
   10 uL KCM buffer<br>
 
   10 uL KCM buffer<br>
 
   70 uL water<br>
 
   70 uL water<br>
 
   1 uL plasmid<br>
 
   1 uL plasmid<br>
#heat shock for 1.5 min at 42 degrees C
+
2. heat shock for 1.5 min at 42 C <br>
#incubate on ice for 5 min.
+
3. incubate on ice for 5 min. <br>
#plate
+
4. plate <br>
 +
-JL_06/09/06

Revision as of 13:05, 9 June 2006

KCM-competent cell heat shock
1. create cocktail on ice

 20 uL TGI cells
10 uL KCM buffer
70 uL water
1 uL plasmid

2. heat shock for 1.5 min at 42 C
3. incubate on ice for 5 min.
4. plate
-JL_06/09/06