Difference between revisions of "IGEM:UC Berkeley/2006/Plasmid Transformation"

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'''KCM-competent cell heat shock'''<br>
 
'''KCM-competent cell heat shock'''<br>
 
#create cocktail on ice
 
#create cocktail on ice
  *20 uL TGI cells
+
*20 uL TGI cells
  *10 uL KCM buffer
+
*10 uL KCM buffer
  *70 uL water
+
*70 uL water
  *1 uL plasmid
+
*1 uL plasmid
 
#heat shock for 1.5 min at 42 degrees C
 
#heat shock for 1.5 min at 42 degrees C
 
#incubate on ice for 5 min.
 
#incubate on ice for 5 min.
 
#plate
 
#plate

Revision as of 12:59, 9 June 2006

KCM-competent cell heat shock

  1. create cocktail on ice
  • 20 uL TGI cells
  • 10 uL KCM buffer
  • 70 uL water
  • 1 uL plasmid
  1. heat shock for 1.5 min at 42 degrees C
  2. incubate on ice for 5 min.
  3. plate