Difference between revisions of "IGEM:UC Berkeley/2006"

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[[Berk2006-ConjugationTeam | Conjugation 6/7/06-7/21/06]]<br>
[[Berk2006-ConjugationTeam | Conjugation 6/7/06-7/21/06]]<br>
[[Berk2006-ConjugationTeam2 | Conjugation 7/24/06 - 09/07/06]]<br>
[[Berk2006-ConjugationTeam2 | Conjugation 7/24/06 - 09/07/06]]<br>
[[Berk2006-ConjugationTeam2 | Conjugation 09/07/06- present]]<br>
[[Berk2006-ConjugationTeam3 | Conjugation 09/07/06- present]]<br>
[[Berk2006-LocksAndKeysTeam | Locks and Keys]]<br>
[[Berk2006-LocksAndKeysTeam | Locks and Keys]]<br>
[[Berk2006-LogicGatesTeam | Logic Gates]]<br>
[[Berk2006-LogicGatesTeam | Logic Gates]]<br>

Revision as of 10:17, 18 September 2006

A Bacterial model for Neural Learning

Information is assimilated in neural systems by an architecture of cell-based switches interconnected by communication channels. These channels are based on physical, spatial connections between cells in the form of dendrite and axons. We are constructing a bacterial model of neural systems where individual bacteria take the place of neurons and communicate with one another through addressable conjugation. Here, bacteria can send messages to one another via conjugation of plasmid DNAs, but the message is only meaningful to cells with a matching address sequence. In this way, the Watson Crick base-pairing of addressing sequences replaces the spatial connectivity present in neural systems. To construct this system, we have adapted natural conjugation systems as the communication device. Information contained in the transferred plasmids is only accessable by "unlocking" the message using RNA based locks and keys. The resulting addressable conjugation process is being adapted to construct a network of NAND logic gates in bacterial cultures. Ultimately, this will allow us to explore different architectures of neural systems to better understand the mechanisms of learning.
GettingStarted iconbaby.png Getting Started on OWW

<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Resources"> </html>Team


Bryan Hernandez
Matt Fleming
Kaitlin A. Davis
Jennifer Lu
Samantha Liang
Daniel Kluesing
Will Bosworth


John Dueber
Chris Anderson

<html> <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> </html>Tools

lab calendar

The Details

Construction Files
-80 Stocks


Procedure Overview (Plasmid DNA to Sequencing)
Plasmid Transformation
Competent cell production
PCR prep
DNA Gel pouring
DNA purifications
PCR machine program (Expand kit)
Knockouts by Electroporation of pOX38 x pKD46

Subgroup Notebooks

Conjugation 6/7/06-7/21/06
Conjugation 7/24/06 - 09/07/06
Conjugation 09/07/06- present
Locks and Keys
Logic Gates
Bryan's Lock and Keys
Samantha's Notebook

<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Resources"> </html>Project

Addressable Cell-to-Cell Communication

Useful Links

Subgroup Strategies, Overview