From OpenWetWare
Revision as of 10:45, 8 June 2006 by JCAnderson (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

Goal: We want to determine the antibiotics resistence of the F and R knockout plasmids (TlambdaOlambda)
We grew TlambdaOlambdaRlambda to early log density. We then streaked the cultures with pipette tips on LB agar plates containing the following anti-biotics:

  1. 15ug/mL gentamicin (G)
  2. 50ug/mL ampicillin (A)
  3. 25ug/mL chloramphenical (C) ---errr, axe that datapoint, the plate disapeared.--JCAnderson 13:45, 8 June 2006 (EDT)
  4. 50ug/mL spectinamycin (Sp)
  5. 25ug/mL tetracycline (Tet)
  6. 20ug/mL trimethylprim (Trim)

TlambdaOlambda grerw on kan only
Rlambda grew on kan and tet only--JCAnderson 19:07, 7 June 2006 (EDT)