Difference between revisions of "IGEM:UC-Merced/2009/Notebook/E. hydro Express"

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Line 43: Line 43:
  
  
 +
10/14/12
  
 +
  Phage
  
 +
1. Overnight culture did not grow.
 +
2. Prepared new stock with frozen bacteria (procedure carried out as on 10/13/12)
 +
    a. LB
 +
    b. Kanomycin to add to strain JW1228-1
 +
        C1V1=C2V2
 +
      25000 ug (V1) = 50ug (5ml)
 +
      C1 = 25000 ug  --> kanomycin concentration
 +
      C2 = 50 ug,  V2 = 5 ml broth
 +
      25000 ug (V1) - 250 ug (ml)
 +
      V1 = 0.01 ml = 10 uL  --> kanomycin to add to strain JW1228-1
 +
      Incubate kanomycin in LB broth until 11am 10/14/12
  
 
      
 
      

Revision as of 17:32, 14 November 2012

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">IGEM:UC-Merced/2009/Notebook/E. hydro Express</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

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Description/Abstract

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Notes

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Lab Notebook

Beginning Tuesday November 13, 2012

10/13/2012

 Phage
  1. Prepared Stocks
   a. LB
   b. Kanomycin to add to strain JW1228-1
       C1V1=C2V2
      25000 ug (V1) = 50ug (5ml)
      C1 = 25000 ug  --> kanomycin concentration
      C2 = 50 ug,   V2 = 5 ml broth
      25000 ug (V1) - 250 ug (ml)
      V1 = 0.01 ml = 10 uL   --> kanomycin to add to strain JW1228-1 
     Incubate kanomycin in LB broth until 11am 10/14/12


10/14/12

 Phage

1. Overnight culture did not grow. 2. Prepared new stock with frozen bacteria (procedure carried out as on 10/13/12)

   a. LB
   b. Kanomycin to add to strain JW1228-1
       C1V1=C2V2
      25000 ug (V1) = 50ug (5ml)
      C1 = 25000 ug  --> kanomycin concentration
      C2 = 50 ug,   V2 = 5 ml broth
      25000 ug (V1) - 250 ug (ml)
      V1 = 0.01 ml = 10 uL   --> kanomycin to add to strain JW1228-1 
     Incubate kanomycin in LB broth until 11am 10/14/12




Recent changes

19 October 2017

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     13:52 

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