Difference between revisions of "IGEM:Stanford/2010/Notebook/15 April 2010"

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(Journal Club 4/15/10)
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The [http://www.nature.com/nature/journal/v403/n6767/abs/403335a0.html original paper].
 
The [http://www.nature.com/nature/journal/v403/n6767/abs/403335a0.html original paper].
[[wikipedia:Repressilator]] on Wikipedia.
+
 
 +
[[wikipedia:Repressilator|Repressilator]] on Wikipedia.
  
 
===See also===
 
===See also===

Revision as of 20:40, 15 April 2010

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Quad center.jpg

Journal Club 4/15/10

Presentation by ...

DNA-ligand binding

d[DNA-L]/dt = k_on*[DNA][L] - k_off*[DNA-L]

If we define: [TotalDNA] = [DNA] + [DNA-L]

Then: d[DNA-L]/dt = k_on*([TotalDNA] - [DNA-L]) - k_off*[DNA-L]

Solving, we get: [DNA-L] = [TotalDNA][L] / (k_off/k_on + L)

Usually, we define the dissociation constant to be: kD = k_off/k_on

So we end up with: [DNA-L] = [TotalDNA][L] / (kD + L)


Other oscillators

Repressilator

Did not end up working too well, but still ground-breaking for its time.

A ---| B ---| C ---| A

The original paper.

Repressilator on Wikipedia.

See also