Difference between revisions of "IGEM:Stanford/2010/Notebook/15 April 2010"

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(DNA-ligand binding)
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==Journal Club 4/15/10==
 
==Journal Club 4/15/10==
 
[http://docs.google.com/present/edit?id=0AeGtz3V56Y9xZGRtOGc0dm1fMTIwNWNqeG03enBn&hl=en Presentation] by ...
 
[http://docs.google.com/present/edit?id=0AeGtz3V56Y9xZGRtOGc0dm1fMTIwNWNqeG03enBn&hl=en Presentation] by ...
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 +
===DNA-ligand binding===
 +
 +
d[DNA-L]/dt = k_on*[DNA][L] - k_off*[DNA-L]
 +
 +
If we define:
 +
[TotalDNA] = [DNA] + [DNA-L]
 +
 +
Then:
 +
d[DNA-L]/dt = k_on*([TotalDNA] - [DNA-L]) - k_off*[DNA-L]
 +
 +
Solving, we get:
 +
[DNA-L] = [TotalDNA][L]/(k_on/k_off + L)

Revision as of 21:25, 15 April 2010

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Quad center.jpg

Journal Club 4/15/10

Presentation by ...

DNA-ligand binding

d[DNA-L]/dt = k_on*[DNA][L] - k_off*[DNA-L]

If we define: [TotalDNA] = [DNA] + [DNA-L]

Then: d[DNA-L]/dt = k_on*([TotalDNA] - [DNA-L]) - k_off*[DNA-L]

Solving, we get:

[DNA-L] = [TotalDNA][L]/(k_on/k_off + L)