IGEM:Stanford/2009/GCPR: Difference between revisions
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*Database of Membrane Protein Structures: http://blanco.biomol.uci.edu/Membrane_Proteins_xtal.html | *Database of Membrane Protein Structures: http://blanco.biomol.uci.edu/Membrane_Proteins_xtal.html | ||
*[http://www.ncbi.nlm.nih.gov/pubmed/15251227 Bible] of GPCR mechanisms. | *[http://www.ncbi.nlm.nih.gov/pubmed/15251227 Bible] of GPCR mechanisms. | ||
*[http://peds.oxfordjournals.org/cgi/reprint/19/1/1 GPCR Engineered in Yeast | *[http://peds.oxfordjournals.org/cgi/reprint/19/1/1 GPCR Engineered in Yeast] | ||
==Questions== | ==Questions== | ||
*I would highly recommend looking through George Georgiou's literature to see what engineered parts he has made. - Chris | *I would highly recommend looking through George Georgiou's literature to see what engineered parts he has made. - Chris | ||
*Thanks for the suggestion; the article for Monday's meeting is by Georgiou. Any other recommendations? | *Thanks for the suggestion; the article for Monday's meeting is by Georgiou. Any other recommendations? | ||
Revision as of 20:11, 4 May 2009
Summary
Comments and Discussion
- Can we identify a specific GPCR or class of GPCRs that we are interested in manipulating?
- Which host organism would make the best chassis?
- What should our GPCR be sensitive to?
Papers
- "Efficient production of membrane-integrated and detergent-soluble G protein-coupled receptors in Escherichia coli," article from Protein Science, 2008: http://www.ncbi.nlm.nih.gov/pubmed/18593817
- Article that discusses some of the complications that emerge when expressing GPCRs in microbial systems. One of the solutions this group found was co-expression of the GPCR-GFP construct with various regulatory proteins involved in membrane metabolism. Coexpression with the AAA+ protease FtsH, an enzyme involvedin MP degradation and quality control. (Article to be read in advance of Monday's meeting)
- "High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1." If we're still interested in veering off towards a medical application, the following article discusses success in expressing various chemokine receptors (CXC motif) in E. coli. These receptors are involved int the immune response, and have implicated in HIV infection and tumor metastasis: http://www.ncbi.nlm.nih.gov/pubmed/19223978?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
- "Characterization of Engineered Channelrhodopsin Variants with Improved Properties and Kinetics," article from Biophysical Journal, March 2009: http://www.cell.com/biophysj/fulltext/S0006-3495(09)00016-2
- Engineering G protein-coupled receptor expression in bacteria, article from PNAS, Sept. 2008: http://www.pnas.org/content/105/39/14747.full
- Database of Membrane Protein Structures: http://blanco.biomol.uci.edu/Membrane_Proteins_xtal.html
- Bible of GPCR mechanisms.
- GPCR Engineered in Yeast
Questions
- I would highly recommend looking through George Georgiou's literature to see what engineered parts he has made. - Chris
- Thanks for the suggestion; the article for Monday's meeting is by Georgiou. Any other recommendations?
