IGEM:PennState/Labbook/NoahJohnson/2007-7-6

July 6, 2007

• Yesterdays motility plates found that motility was greatest at 26°C so today we are running 5 motility trials at 26°C with varying concentrations of eiken agar
• Made motility plates
  • 0.25g tryptone
  • 0.2g NaCl
  • 0.15g sodium citrate
  • 25mL water
  • Eiken Agar:
    • 4 plates of 0.24%
    • 4 plates of 0.26%
    • 4 plates of 0.28%
    • 4 plates of 0.30%
    • 4 plates of 0.32%

• Ran Gel on Pfx PCR products (Start 2:00)
• Used Sybr-green
  • Well 1- DNA Ladder
  • Well 2- (-) Pfx
  • Well 3- 56°C Pfx
  • Well 4- 57.1°C Pfx
  • Well 5- 58.6°C Pfx
  • Well 6- 61.3°C Pfx
  • Well 7- 64.9°C Pfx
  • Well 8- 67.5°C Pfx
  • Well 9- 69.1°C Pfx
  • Well 10- 70°C Pfx