IGEM:PennState/Labbook/NoahJohnson

<calendar> name=iGEM:MIT/2006/Notebook date=2007/04/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

<calendar> name=iGEM:MIT/2006/Notebook date=2007/07/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

<calendar> name=iGEM:MIT/2006/Notebook date=2007/10/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

5-24-07

Restriction Digest

1. Insert A,B,C
   • 3.5 H20
   • 3.5 DNA (J23038 A,B,C)
   • 1 EcoR1 Buf
   • 1 BSA
   • 0.5 EcoR1
   • 0.5 SpeI

1. Vector A,B,C
   • 3.5 H20
   • 3.5 DNA (J31004 A,B,C)
   • 1 EcoR1 Buf
   • 1 BSA
   • 0.5 EcoR1
   • 0.5 XbaI

1. Vector -
   • 6.5 H2O
   • 3.5 DNA (J31004A)

1. EcoR1 Vector
   • 4 H2O
   • 3.5 DNA (J31004 A)
   • 1 EcoR1 Buf
   • 1 BSA
   • 0.5 EcoR1

1. XbaI Vector
   • 4 H2O
   • 3.5 DNA (J31004 A)
   • 1 Buffer 2
   • 1 BSA
   • 0.5 Xba

1. SpeI Vector
   • 4 H2O
   • 3.5 DNA (J31004 A)
   • 1 Buffer 2
   • 1 BSA
   • 0.5 SpeI