IGEM:PennState/Labbook/NoahJohnson

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<calendar> name=iGEM:MIT/2006/Notebook date=2007/04/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

<calendar> name=iGEM:MIT/2006/Notebook date=2007/07/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

<calendar> name=iGEM:MIT/2006/Notebook date=2007/10/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

5-24-07

Restriction Digest

  1. Insert A,B,C
    • 3.5 H20
    • 3.5 DNA (J23038 A,B,C)
    • 1 EcoR1 Buf
    • 1 BSA
    • 0.5 EcoR1
    • 0.5 SpeI
  1. 2Vector A,B,C
    • 3.5 H20
    • 3.5 DNA (J31004 A,B,C)
    • 1 EcoR1 Buf
    • 1 BSA
    • 0.5 EcoR1
    • 0.5 XbaI
  1. Vector -
    • 6.5 H2O
    • 3.5 DNA (J31004A)
  1. EcoR1 Vector
    • 4 H2O
    • 3.5 DNA (J31004 A)
    • 1 EcoR1 Buf
    • 1 BSA
    • 0.5 EcoR1
  1. XbaI Vector
    • 4 H2O
    • 3.5 DNA (J31004 A)
    • 1 Buffer 2
    • 1 BSA
    • 0.5 Xba
  1. SpeI Vector
    • 4 H2O
    • 3.5 DNA (J31004 A)
    • 1 Buffer 2
    • 1 BSA
    • 0.5 SpeI