Difference between revisions of "IGEM:PennState/Labbook/Nimrah Ahmed"

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==Description/Abstract==
 
==Description/Abstract==
* Place short description of project or notes regarding this project
+
Currently working on finding a project
  
  
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==Meeting 5/15/2008==
 
===Circuit project ideas:===
 
* Genetic Circuit basics: http://www.princeton.edu/~rweiss/bio-programming/weiss-natural-computing-2003.pdf
 
====Sequential cell color change====
 
*ex. Green->Red->Yellow->repeat.
 
*System: organic layer & bacterial aquatic layer in liquid form.  Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
 
*Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
 
 
=====Components=====
 
* Organic compound: hydrophobic, heavy (won't evaporate), is recognized by cell
 
* Recognition compound(s): recognize organic compound and synthesize/activate something that activates promoter
 
* Promoter: probably lac
 
* Color proteins
 
 
=====Circuit=====
 
 
O -> A
 
 
A+B -> C, GFP
 
 
A+C -> B, YFP
 
 
 
*Degradation tags on A, B, C, GFP, YFP, strongest on A, weakest on B and C.
 
*Intended result: shake once: GFP, shake again: YFP, shake again, GFP, etc.
 
 
=====Options:=====
 
*Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...
 
*Rather than shake, stir by '''thermal convection''' or a '''magnetic stirrer'''
 
 
(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)
 
 
 
[[User:Nimrah Ahmed|Nimrah Ahmed]] 12:26, 15 May 2008 (EDT)
 
 
 
==Work Day Monday 5/19/2008==
 
*Introduction to lab & equipment
 
===Making Media===
 

Revision as of 12:30, 20 May 2008

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Description/Abstract

Currently working on finding a project


Notes

  • Team meetings: Monday 5:00pm
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