Difference between revisions of "IGEM:PennState/Labbook/Nimrah Ahmed"

From OpenWetWare
Jump to: navigation, search
m (5/19)
m (cleanup)
Line 14: Line 14:
* Place short description of project or notes regarding this project
Currently working on finding a project
Line 29: Line 29:
==Meeting 5/15/2008==
===Circuit project ideas:===
* Genetic Circuit basics: http://www.princeton.edu/~rweiss/bio-programming/weiss-natural-computing-2003.pdf
====Sequential cell color change====
*ex. Green->Red->Yellow->repeat.
*System: organic layer & bacterial aquatic layer in liquid form.  Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
*Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
* Organic compound: hydrophobic, heavy (won't evaporate), is recognized by cell
* Recognition compound(s): recognize organic compound and synthesize/activate something that activates promoter
* Promoter: probably lac
* Color proteins
O -> A
A+B -> C, GFP
A+C -> B, YFP
*Degradation tags on A, B, C, GFP, YFP, strongest on A, weakest on B and C.
*Intended result: shake once: GFP, shake again: YFP, shake again, GFP, etc.
*Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...
*Rather than shake, stir by '''thermal convection''' or a '''magnetic stirrer'''
(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)
[[User:Nimrah Ahmed|Nimrah Ahmed]] 12:26, 15 May 2008 (EDT)
==Work Day Monday 5/19/2008==
*Introduction to lab & equipment
===Making Media===

Revision as of 12:30, 20 May 2008

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext">05/15/2008,05/20/2008,05/22/2008,05/27/2008,05/28/2008</div><div style="display:none;" id="page">IGEM:PennState/Labbook/Nimrah Ahmed</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

Igem-logo-150px.png <sitesearch>title=Search this Project</sitesearch>

Customize your entry pages <html><img src="/images/a/aa/Help.png" border="0" /></html>


Currently working on finding a project


  • Team meetings: Monday 5:00pm
Recently Edited Notebook Pages