IGEM:Peking University/2008/Experiment/PKU 080527 G1 1

PKU_080527_G1_1

---

PCR- pGREG504/(pESC-hAID)-hAID-50ul

	Volume (μL)
pGREG504/(pESC-hAID)	1
transtart taq	1
transtart buffer	5
dNTP	3
F-Primer	1
R-Primer	1
ddH2O	58

conditions

Temperature(°C)	Time Length(0:00:00)
94	04:00
50	02:00
72	02:00
94	01:00	30 cycles
56	02:00	30 cycles
72	01:00	30 cycles
72	05:00

---

Enzyme cutting-pGREG506'/(pADH-lacO1)-ClaI-BamHI

	Volume (μL)
pGREG506'/(pADH-lacO1)	5
ClaI	0.2
BamHI	0.2
K Buffer	2
ddH2O	12.6

didn't work

back to Previous Page