Difference between revisions of "IGEM:Peking University/2008/Experiment/PKU 080519 G1 1"

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(PKU_080519_G1_1)
(PKU_080519_G1_1)
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|BamHI||0.2||
 
|BamHI||0.2||
 
|-  
 
|-  
|H Buffer||2
+
|K Buffer||2
 
|-
 
|-
 
|ddH<sub>2</sub>O||13.6  
 
|ddH<sub>2</sub>O||13.6  

Revision as of 03:29, 4 July 2008

PKU_080519_G1_1


Cultivation- E.Coli(liguid-Amp+)

Plasmid Miniprep-pGREG504/pADH

Enzyme cutting-pGREG504/pADH-EcoRV
File:PKU 080519 check-pGREG504-pADH.tif
Volume (μL)
pGREG504/pADH 5
EcoRV 0.2 *13
H Buffer 2
ddH2O 12.8


Enzyme cutting-pGREG504/pADH-SacI-BamHI
Volume (μL)
pGREG504/pADH 5
SacI 0.2 *13
BamHI 0.2
K Buffer 2
ddH2O 13.6


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