Difference between revisions of "IGEM:Peking University/2008/Experiment/PKU 080519 G1 1"

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(New page: ==PKU_080519_G1_1== <hr class=divider> ::'''Cultivation- E.Coli(liguid-Amp<sup>+</sup>)''' <hr class=divider> ::'''Plasmid Miniprep-pGREG504/pADH''' <hr class=divider> ::'''Enzyme cutting-...)
 
(PKU_080519_G1_1)
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<hr class=divider>
 
<hr class=divider>
 
::'''Cultivation- E.Coli(liguid-Amp<sup>+</sup>)'''
 
::'''Cultivation- E.Coli(liguid-Amp<sup>+</sup>)'''
 +
 
<hr class=divider>
 
<hr class=divider>
 
::'''Plasmid Miniprep-pGREG504/pADH'''
 
::'''Plasmid Miniprep-pGREG504/pADH'''
 +
 
<hr class=divider>
 
<hr class=divider>
 
::'''Enzyme cutting-pGREG504/pADH-EcoRV'''
 
::'''Enzyme cutting-pGREG504/pADH-EcoRV'''
 
{|align="right"
 
{|align="right"
PKU_
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[[PKU_20080512_adhpcrcheck.tif|200px]]
 
|}
 
|}
 
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Revision as of 08:33, 3 July 2008

PKU_080519_G1_1


Cultivation- E.Coli(liguid-Amp+)

Plasmid Miniprep-pGREG504/pADH

Enzyme cutting-pGREG504/pADH-EcoRV
200px
Volume (μL)
pGREG504/pADH 5
EcoRV 0.2 *13
H Buffer 2
ddH2O 12.8


Enzyme cutting-pGREG504/pADH-SacI-BamHI
Volume (μL)
pGREG504/pADH 5
SacI 0.2 *13
BamHI 0.2
H Buffer 2
ddH2O 13.6