IGEM:Peking University/2008/Experiment/PKU 080513 G1 1: Difference between revisions

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==PKU_080513_G1_1==
==PKU_080513_G1_1==
<hr class=divider>
<hr class=divider>
::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul'''
::'''PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul'''
{|align="right"
{|align="right"
[[PKU_20080513_adhpcrcheck.tif|200px]]
[[PKU_20080513_adhpcrcheck.tif|200px]]
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|+  
|+  
|-
|-
|Temperature(&&&)||Time Length(0:00:00)
|Temperature(°C)||Time Length(0:00:00)
|-  
|-  
| 94||05:00
| 94||05:00
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| Alarm||5 times
| Alarm||5 times
|-
|-
| Hold||4&&&
| Hold||4°C
|}
|}


Revision as of 09:53, 3 July 2008

PKU_080513_G1_1

PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul
200px
Volume (μL)
pGREG505/(TEF-lacI-adhlacS-GFP) 1
dNTP 5
transtart taq 1
transtart Buffer 5
F-Primer 1
R-Primer 1
ddH2O 36


conditions
Temperature(°C) Time Length(0:00:00)
94 05:00
94 00:30 30 cycles
50,55,60 00:30 30 cycles
72 01:30 30 cycles
72 07:00
Alarm 5 times
Hold 4°C



Enzyme cutting-DNA (pGREG503/504)-SacI-XhoI-20 μL
200px
Volumn(μL)
pGREG503/504 5
SacI 0.2
XhoI 0.2 *5
M Buffer 2
ddH2O 12.6
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