Difference between revisions of "IGEM:Peking University/2008/Experiment/PKU 080512 G1 1"

From OpenWetWare
Jump to: navigation, search
(PKU_080512_G1_1)
(PKU_080512_G1_1)
Line 72: Line 72:
 
|-
 
|-
 
| Hold||4°C
 
| Hold||4°C
 +
|}
 +
 +
{|align="right"
 +
|back to [[PKU_Experiment|Previous Page]]
 
|}
 
|}

Revision as of 08:42, 3 July 2008

PKU_080512_G1_1


Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
200px
Volume (μL)
DNA 5
ClaI 0.2
BamH1 0.2
KBuffer 2
ddH2O 12.6



PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
200px
Volume (μL)
pGREG505/(TEF-lacI-adhlacS-GFP) 5
dNTP 5
KOD Plus 1
KOD Plus Buffer 5
MgSO4 2
F-Primer 1
R-Primer 1
ddH2O 34


conditions
Temperature(°C) Time Length(0:00:00)
94 05:00
94 00:30 30 cycles
55 00:30 30 cycles
72 01:30 30 cycles
72 07:00
Alarm 5 times
Hold 4°C
back to Previous Page