Difference between revisions of "IGEM:Peking University/2008/Experiment/PKU 080512 G1 1"

From OpenWetWare
(PKU_080512_G1_1)
(PKU_080512_G1_1)
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|Temperature(&&&)||Time Length(0:00:00)
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|Temperature(°C)||Time Length(0:00:00)
 
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| 94||05:00
 
| 94||05:00
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| Alarm||5 times
 
| Alarm||5 times
 
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| Hold||4&&&
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| Hold||4°C
 
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Revision as of 08:41, 3 July 2008

PKU_080512_G1_1


Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
200px
Volume (μL)
DNA 5
ClaI 0.2
BamH1 0.2
KBuffer 2
ddH2O 12.6



PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
200px
Volume (μL)
pGREG505/(TEF-lacI-adhlacS-GFP) 5
dNTP 5
KOD Plus 1
KOD Plus Buffer 5
MgSO4 2
F-Primer 1
R-Primer 1
ddH2O 34


conditions
Temperature(°C) Time Length(0:00:00)
94 05:00
94 00:30 30 cycles
55 00:30 30 cycles
72 01:30 30 cycles
72 07:00
Alarm 5 times
Hold 4°C