Difference between revisions of "IGEM:Peking/2007/Switch-Notebook/2007-8-16"
Latest revision as of 01:48, 18 August 2007
Miniprep and double digestion of SulA, lac, SS3, SD1-2, Rec-(GFP-pLX007)
Double digested with XhoI/SalI
10uL Plasmid 2uL 10*H buffer 0.25uL XhoI 0.25uL SalI 7.5uL ddH2O ------------------ 20uL total
Run electrophoresis and send the correct plasmid for sequencing
SulA, lac, Rec, SS3, SD2 are sent.
SD1 are found wrong.