Difference between revisions of "IGEM:Peking/2007/Switch-Notebook/2007-8-14"

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Line 13: Line 13:
 
Number of colonies grew up:
 
Number of colonies grew up:
 
<pre>
 
<pre>
Prec 0
+
      No.
SD2   0
+
----------
SD3   1
+
Prec   0
SS1 >50
+
SD2     0
SS2   2
+
SD3     1
SS3 14
+
SS1   >50
 +
SS2     2
 +
SS3   14
 
</pre>
 
</pre>
  
 
Only SS1 is right. 4 colonies are select for incubation.
 
Only SS1 is right. 4 colonies are select for incubation.

Revision as of 23:06, 17 August 2007

Ligation of SulA, Rec, SD1-3, SS1-2 with GFP-pLX007

Precipitate for retraction

SulA, Rec, SD1-3, SS1-2, GFP-pLX007 (^^ with XbaI & XhoI)

Ligate with GFP-pLX007

SulA, Rec, SD1-3, SS1-2

Transformed in the evening

Colony PCR test of Prec, SD2-3, SS1-3-(SDY-EGFP-pLX007)

Number of colonies grew up:

       No.
----------
Prec    0
SD2     0
SD3     1
SS1   >50
SS2     2
SS3    14

Only SS1 is right. 4 colonies are select for incubation.