IGEM:Peking/2007/Switch-Notebook/2007-7-17

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Experimenter: LCB, CXY

Contents

Electrophoresis for SD2

Re-do temperature PCR of SD2

Re-Re-do temperature PCR of CD2, third time(!!!) by NM

verify the concentration of LacZa(XhoI/SalI)

  • got by Bobo on 9/7/07

too weak!.

digesting pLX007(1-6) with SalI or XhoI, respectively

double enzymes digest pLX007, pLX008 and pLX009 for getting vector

PCR for rec promoter

  • Genome of GM1655 as temple