IGEM:Peking/2007/Count:Gel-Extraction: Difference between revisions
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Qu Mingzhi (talk | contribs) (New page: ==quick gel purifcation== according to ''Transgen'' kit protocol #Agarose gel electrophoresis,cut the DNA band as thin as possible.pute into a clear EP tube.weighing,if the weight is 100mg...) |
Qu Mingzhi (talk | contribs) |
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==quick gel purifcation== | ==quick gel purifcation== | ||
according to ''Transgen'' kit protocol | according to ''Transgen'' kit protocol | ||
#Agarose gel electrophoresis,cut the DNA band as thin as possible.pute into a clear EP tube.weighing,if the weight is 100mg,the volume can be | #Agarose gel electrophoresis,cut the DNA band as thin as possible.pute into a clear EP tube.weighing,if the weight is 100mg,the volume can be considered as 100uL,add solution GSB,put into 55C water bath until complently merge,it takes about 5 minutes | ||
#cool the tube with water to adjust the tempertature of the solution to room temperature | #cool the tube with water to adjust the tempertature of the solution to room temperature | ||
#Add the solution into the collection tube, standing for 1 minute,13000rpm for 1 min, discard the flow-through | #Add the solution into the collection tube, standing for 1 minute,13000rpm for 1 min, discard the flow-through | ||
Latest revision as of 09:36, 9 July 2007
quick gel purifcation
according to Transgen kit protocol
- Agarose gel electrophoresis,cut the DNA band as thin as possible.pute into a clear EP tube.weighing,if the weight is 100mg,the volume can be considered as 100uL,add solution GSB,put into 55C water bath until complently merge,it takes about 5 minutes
- cool the tube with water to adjust the tempertature of the solution to room temperature
- Add the solution into the collection tube, standing for 1 minute,13000rpm for 1 min, discard the flow-through
- Add 650 uL WB,13000rpm for 1 min,discard the flow-through
- 13000rpm for 2 min,discard the flow-through
- Put the collection tube into a clean EP tube, Add 50 uL 60C ddH2O in the center of the collection tube,standing for 1 min
- 13000rpm for 1 min.
- store the DNA solution at -20C
