Difference between revisions of "IGEM:Peking/2007/Count:Gel-Extraction"

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(New page: ==quick gel purifcation== according to ''Transgen'' kit protocol #Agarose gel electrophoresis,cut the DNA band as thin as possible.pute into a clear EP tube.weighing,if the weight is 100mg...)
 
(quick gel purifcation)
 
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==quick gel purifcation==
 
==quick gel purifcation==
 
according to ''Transgen'' kit protocol
 
according to ''Transgen'' kit protocol
#Agarose gel electrophoresis,cut the DNA band as thin as possible.pute into a clear EP tube.weighing,if the weight is 100mg,the volume can be  
+
#Agarose gel electrophoresis,cut the DNA band as thin as possible.pute into a clear EP tube.weighing,if the weight is 100mg,the volume can be considered as 100uL,add solution GSB,put into 55C water bath until complently merge,it takes about 5 minutes
 
 
consider as 100uL,add solution GSB,put into 55C water bath until complently merge,it takes about 5 minutes
 
 
#cool the tube with water to adjust the tempertature of the solution to room temperature
 
#cool the tube with water to adjust the tempertature of the solution to room temperature
 
#Add the solution into the collection tube, standing for 1 minute,13000rpm for 1 min, discard the flow-through
 
#Add the solution into the collection tube, standing for 1 minute,13000rpm for 1 min, discard the flow-through

Latest revision as of 08:36, 9 July 2007

quick gel purifcation

according to Transgen kit protocol

  1. Agarose gel electrophoresis,cut the DNA band as thin as possible.pute into a clear EP tube.weighing,if the weight is 100mg,the volume can be considered as 100uL,add solution GSB,put into 55C water bath until complently merge,it takes about 5 minutes
  2. cool the tube with water to adjust the tempertature of the solution to room temperature
  3. Add the solution into the collection tube, standing for 1 minute,13000rpm for 1 min, discard the flow-through
  4. Add 650 uL WB,13000rpm for 1 min,discard the flow-through
  5. 13000rpm for 2 min,discard the flow-through
  6. Put the collection tube into a clean EP tube, Add 50 uL 60C ddH2O in the center of the collection tube,standing for 1 min
  7. 13000rpm for 1 min.
  8. store the DNA solution at -20C