Difference between revisions of "IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/07/25"

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<font color=red>Antoine</font>
 
<font color=red>Antoine</font>
  
 +
<br><u>Minipreps</u>
 +
*B0015 272.5 ng/μL
 +
*J23110 991.9 ng/μL
  
 +
<br><u>Restriction digest</u>
 +
* B0015 cut by X+P: 25 μL miniprep (22/07) + 1 μL each enzyme + 0.3 μL BSA + 3 μL Buffer 3
 +
* P1003 cut by S+P: 6 μL miniprep (22/07) + 1 μL each enzyme + 0.1 μL BSA + 1 μL Buffer 2 + 0.9 H20
 +
* J23110 cut by S+P: 16 μL miniprep (22/07) + 1 μL each enzyme + 0.2 μL BSA + 2 μL Buffer 2
 +
* C0040 cut by X+P: 9 μL miniprep (22/07) + 1 μL each enzyme + 0.15 μL BSA + 1.5 μL Buffer 3 + 2.35 H20
 +
* C0012 cut by X+P: 19 μL miniprep (22/07) + 1 μL each enzyme + 0.25 μL BSA + 2.5 μL Buffer 3 + 1.25 H2O
 +
 +
Inactivation 20 min 65°C and storage at -20°C
 
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Population counter</span>
 
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Population counter</span>

Revision as of 04:24, 2 August 2010

Igem-logo-150px.pngMemo cell <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Antoine


Minipreps

  • B0015 272.5 ng/μL
  • J23110 991.9 ng/μL


Restriction digest

  • B0015 cut by X+P: 25 μL miniprep (22/07) + 1 μL each enzyme + 0.3 μL BSA + 3 μL Buffer 3
  • P1003 cut by S+P: 6 μL miniprep (22/07) + 1 μL each enzyme + 0.1 μL BSA + 1 μL Buffer 2 + 0.9 H20
  • J23110 cut by S+P: 16 μL miniprep (22/07) + 1 μL each enzyme + 0.2 μL BSA + 2 μL Buffer 2
  • C0040 cut by X+P: 9 μL miniprep (22/07) + 1 μL each enzyme + 0.15 μL BSA + 1.5 μL Buffer 3 + 2.35 H20
  • C0012 cut by X+P: 19 μL miniprep (22/07) + 1 μL each enzyme + 0.25 μL BSA + 2.5 μL Buffer 3 + 1.25 H2O

Inactivation 20 min 65°C and storage at -20°C

Igem-logo-150px.png Population counter <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Raphaël
Glycerol 15% 2 glycerols per culture, one stored at -20°C and the other at -80°C

  • TOP10 pSB1A2 weak RBS -TetA(C) - Amp
  • TOP10 pSB1A2 med.RBS -TetA(C) - Amp
  • TOP10 pSB1A2 weak RBS -LuxR - Amp
  • TOP10 pSB1A2 med.RBS -LuxR - Amp
  • TOP10 pSB1A2 stand.RBS -LuxR - Amp


Minipreps : 4 minipreps of a 10ml culture per biobrick, eluted with 30μL buffer and collected into 1 tube for a total volume of around 120μL:

  • weak RBS -TetA(C) - Amp
  • med.RBS -TetA(C) - Amp
  • weak RBS -LuxR - Amp
  • med.RBS -LuxR - Amp
  • stand.RBS -LuxR - Amp


Nanodrop to determine the amount of DNA in the miniprep samples:

Sample name 260/280 260/230 ng/μL
weak RBS -TetA(C) 1.87 2.31 186.5
med.RBS -TetA(C) 1.86 2.31 185.1
weak RBS -LuxR 1.84 2.38 159.8
med.RBS -LuxR 1.84 2.36 175.6
stand.RBS -LuxR 1.85 2.35 191.7