IGEM:Paris Bettencourt 2012/Protocols/Plates

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Plate color codes

Antibiotic type
Color code
Dilutant
Stock concentration
Dilution
Plain
black
-
-
-
Ampicilline
red
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100 mg/ml
x1000
Chloramphenicol
blue
ethanol 100%
30 mg/ml
x1000
Kanamycine
green
Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle H_{2}O} + filtration
100 mg/ml
x1000
Spectinomycine
orange
Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle H_{2}O} + filtration
25 mg/ml
x500/333

LB Agar (LBA) Protocol (500ml)

  • Melt LBA in microwave (~8 min).
  • Cool LBA before you can touch it without burn your skin (~4 min in water bath).
  • Add required chemicals (antibiotic, etc).
  • Add 24 ml of LBA into each plate.
  • Wait while plates will be thicken.
  • Dry them 45' under the flow hood, with the lid open.