Difference between revisions of "IGEM:Paris Bettencourt 2012/Protocols/Biobrick Retrieval"

From OpenWetWare
Jump to: navigation, search
 
Line 1: Line 1:
[[File:Biobrick_deposit.png|thumb|left|alt=Red DNA stripe|Dry DNA and red dye is deposited along one corner of the well. Find the DNA's location before you add water.]]
+
==Biobrick Retrieval==
 +
This protocol can be used to retrieve biobrick DNA from kit plates. The resulting DNA solution can be used directly for transformation.
 +
 
 +
* Find the correct Plate and well by locating the kit edition (year), plate number, and well coordinate (i.e. 2012 Kit Plate 4 Well 1A)
 +
* Look at your well from below to find the where the DNA is deposited. It is visible as a deposit of red solid at one of the corners (see image).
 +
* Punch a hole in the foil of your well using a 200 uL pipette tip. Push the foil to the sides.
 +
* Using another 200 uL pipette tip, carefully add 10 uL of distilled water to the well, on the DNA.
 +
* Gently scrape and mix the DNA. Pipette up and down.
 +
* Let the solution incubate in the well at least 5 minutes.
 +
* Transfer the 10 uL of DNA solution to a 1.5 mL centrifuge tube.
 +
* Store DNA in -20C.
 +
 
 +
[[Image:Biobrick_deposit.png|thumb|left|alt=Red DNA stripe|Dry DNA and red dye is deposited along one corner of the well. Find the DNA's location before you add water.]]

Revision as of 05:39, 5 July 2012

Biobrick Retrieval

This protocol can be used to retrieve biobrick DNA from kit plates. The resulting DNA solution can be used directly for transformation.

  • Find the correct Plate and well by locating the kit edition (year), plate number, and well coordinate (i.e. 2012 Kit Plate 4 Well 1A)
  • Look at your well from below to find the where the DNA is deposited. It is visible as a deposit of red solid at one of the corners (see image).
  • Punch a hole in the foil of your well using a 200 uL pipette tip. Push the foil to the sides.
  • Using another 200 uL pipette tip, carefully add 10 uL of distilled water to the well, on the DNA.
  • Gently scrape and mix the DNA. Pipette up and down.
  • Let the solution incubate in the well at least 5 minutes.
  • Transfer the 10 uL of DNA solution to a 1.5 mL centrifuge tube.
  • Store DNA in -20C.
Red DNA stripe
Dry DNA and red dye is deposited along one corner of the well. Find the DNA's location before you add water.