IGEM:Paris Bettencourt 2012/Protocols/Biobrick Retrieval: Difference between revisions
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[[ | ==Biobrick Retrieval== | ||
This protocol can be used to retrieve biobrick DNA from kit plates. The resulting DNA solution can be used directly for transformation. | |||
* Find the correct Plate and well by locating the kit edition (year), plate number, and well coordinate (i.e. 2012 Kit Plate 4 Well 1A) | |||
* Look at your well from below to find the where the DNA is deposited. It is visible as a deposit of red solid at one of the corners (see image). | |||
* Punch a hole in the foil of your well using a 200 uL pipette tip. Push the foil to the sides. | |||
* Using another 200 uL pipette tip, carefully add 10 uL of distilled water to the well, on the DNA. | |||
* Gently scrape and mix the DNA. Pipette up and down. | |||
* Let the solution incubate in the well at least 5 minutes. | |||
* Transfer the 10 uL of DNA solution to a 1.5 mL centrifuge tube. | |||
* Store DNA in -20C. | |||
[[Image:Biobrick_deposit.png|thumb|left|alt=Red DNA stripe|Dry DNA and red dye is deposited along one corner of the well. Find the DNA's location before you add water.]] |
Revision as of 05:39, 5 July 2012
Biobrick Retrieval
This protocol can be used to retrieve biobrick DNA from kit plates. The resulting DNA solution can be used directly for transformation.
- Find the correct Plate and well by locating the kit edition (year), plate number, and well coordinate (i.e. 2012 Kit Plate 4 Well 1A)
- Look at your well from below to find the where the DNA is deposited. It is visible as a deposit of red solid at one of the corners (see image).
- Punch a hole in the foil of your well using a 200 uL pipette tip. Push the foil to the sides.
- Using another 200 uL pipette tip, carefully add 10 uL of distilled water to the well, on the DNA.
- Gently scrape and mix the DNA. Pipette up and down.
- Let the solution incubate in the well at least 5 minutes.
- Transfer the 10 uL of DNA solution to a 1.5 mL centrifuge tube.
- Store DNA in -20C.