IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/28: Difference between revisions

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===Colicin Assay===
===Colicin Assay===
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Revision as of 04:35, 17 September 2012

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Colicin Assay

Plate Lawn (20 uL) Spot (5uL)
LB MG1655 MG1655
LB MG1655 WT ColE2
LB MG1655 Z1 pSG.008 WT ColE2
LB NEB Turbo +pSG.008 WT ColE2
LB+IPTG MG1655 Z1 pSG.008 WT ColE2
LB+IPTG NEB Turbo +pSG.008 WT ColE2

Ligation

Reagent pSG.025A pSG.025B pSG.018 S,P -
Vector 3 uL (pSG.018 S,P) 3 uL (pSG.018 S,P) 3 uL (pSG.018 S,P)
Insert 5.5 uL (pSG.013A X,P) 5.5 uL (pSG.013B X,P) -
Buffer 10X 1 uL 1 uL 1 uL
T4 DNA ligase 0.5 uL 0.5 uL 0.5 uL
H2O - - 5.5 uL
Total 10 uL 10 uL 10 uL

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