Difference between revisions of "IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/15"

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Revision as of 04:37, 17 September 2012

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Restriction Digest

-Digest vectors to be ligated with constitutive promoters and pTet

Reagent pSG.001 pSG.002
Plasmid 10 uL 7 uL
Green Buffer 10X 4 uL 4uL
SpeI 2uL 2uL
EcorI 2uL 2uL
H2O 22uL 27uL
Total 40uL 40uL

Results

SGgels1508121.jpg

SGgel1508122.jpg

Ligation Reactions

Vector Insert Product Colonies
pSG.003 (S,P)

pLac, pSB1A2

+pSG.014-OS (X,P)

ColE2 Imm

→ pSG.021 OS

(AMP)

++
pSG.003 (S,P)

pLac, pSB1A2

+pSG.014-BB (X,P)

ColE2 Imm

→ pSG.021 BB

(AMP)

++
pSG.005 (S,P)

pLac, pSB3C5

+pSG.014-OS (X,P)

ColE2 Imm

→ pSG.008 OS

(Cm)

++
pSG.005 (S,P)

pLac, pSB3C5

+pSG.014-BB (X,P)

ColE2 Imm

→ pSG.008 BB

(Cm)

++
pSG.003

pLac, pSB1A2

- pSG.003

pLac, pSB1A2

+
pSG.005

pLac, pSB3C5

- pSG.005

pLac, pSB3C5

+
pSG.001 (E,S)

pSB3C5

+pSG.015 (An)

pCons.86

→ pSG.017

(Cm)

++
pSG.002 (E,S)

pSB4K5

+pSG.016 (An)

pCons.51

→ pSG.018

(Kan)

++
pSG.002 (E,S)

pSB4K5

+pSG.004 (An)

pTet

→ pSG.006

(Kan)

-
pSG.002 (E,S)

pSB4K5

- - -
pSG.002

pSB4K5

- pSG.002

pSB4K5

+