Difference between revisions of "IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/10"

From OpenWetWare
Jump to: navigation, search
Line 43: Line 43:
So ok, it is NOT the immunity gene.
So ok, it is NOT the immunity gene.

Revision as of 14:36, 24 September 2012

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Digestion of pSG.021(?)

  • We did digestion of pSG.021 (pLac - ColE2Imm) plasmids from the 2nd colony with X and P.
  • The expected size of insert is about 300bp.


Plasmid 6 uL
H2O 26 uL
FD 10x Green Buffer 4 uL
XbaI 2 uL
PstI 2 uL
Total 40 uL

Incubate 1h15 with shaking at 37°C.



... So ok, it is NOT the immunity gene.