Difference between revisions of "IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/20"

From OpenWetWare
Jump to: navigation, search
(Entry title)
(Analytical digest of pMG.004 round 3)
Line 29: Line 29:
  
 
==Analytical digest of pMG.004 round 3==
 
==Analytical digest of pMG.004 round 3==
*To discover is there is bad XbaI or bad plasmid
+
*To discover if there is bad XbaI or bad plasmid
  
 
{| class="wikitable"
 
{| class="wikitable"

Revision as of 09:00, 20 July 2012

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Transformation with new TOP10 CaCl2 competent cells from 19/07/12

  • 1 uL of DNA added to each
Plate Plasmid Concentration DNA [ng/uL] Volume plated [uL]
1 pSB1C3 100 200
2 pSB1C3 10 200
3 pSB1C3 100 200
4 pSB1C3 10 200
5 pMG.004 202 200
6 pMG.004 202 20

Analytical digest of pMG.004 round 3

  • To discover if there is bad XbaI or bad plasmid
Plate Plasmid
XbaI tube 1 + PstI pMG.004 2uL
XbaI tube 2 + PstI pMG.004 2uL
XbaI tube 3 + PstI pMG.004 2uL
XbaI tube 4 + PstI pMG.004 2uL
NotI pMG.004 2uL