Difference between revisions of "IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/17"

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(Entry title)
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==Entry title==
+
==Biobrick BBa_I716462==
 
in accordance with discussion with Yifan,
 
in accordance with discussion with Yifan,
 
we want to use the BamH1Bekerley 2007/ Letherbridge 2011 project.[[ LINK]]
 
we want to use the BamH1Bekerley 2007/ Letherbridge 2011 project.[[ LINK]]
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( it is registered in the "online following sheet")
 
( it is registered in the "online following sheet")
  
 +
==Transformation==
  
 +
* of pMG.004, AKA BBa_I716462
 +
* Following jake's protocol
 +
 +
{| class="wikitable"
 +
{| border="1"
 +
|-
 +
! Plate !! Plasmid !! Dilution
 +
|-
 +
| 1 || BBa_I716462 from registry || 1:1
 +
|-
 +
| 2 || BBa_I716462 from registry || 1:10
 +
|-
 +
| 3 || lacZ on pSB1A2 108 ng/uL  || 1:1
 +
|-
 +
| 4 || lacZ on pSB1A2 108 ng/uL  || 1:10
 +
|}
  
 
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Revision as of 09:02, 17 July 2012

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Biobrick BBa_I716462

in accordance with discussion with Yifan, we want to use the BamH1Bekerley 2007/ Letherbridge 2011 project.LINK

opening of a new biobrick:

Code Date Received Registry Markers Rep. Origin Backbone Source Description of Function Location Notes
pMG.004 17-07-12 BBa_I716462 A pMB1 pSB1A2 2012 Kit Plate 2 3C BamHI endonuclease under pBAD

( it is registered in the "online following sheet")

Transformation

  • of pMG.004, AKA BBa_I716462
  • Following jake's protocol
Plate Plasmid Dilution
1 BBa_I716462 from registry 1:1
2 BBa_I716462 from registry 1:10
3 lacZ on pSB1A2 108 ng/uL 1:1
4 lacZ on pSB1A2 108 ng/uL 1:10