Difference between revisions of "IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/09"

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(Autocreate 2012/07/09 Entry for IGEM:Paris_Bettencourt_2012/Notebooks/modularity_group/day_by_day/)
 
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==Entry title==
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==Luminescence test of pMG.002==
* Insert content here...
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* Re-doing test for visible luminescence. NO ANTIBIOTIC ADDED
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* 20 mL LB + [100 uM, 1 mM] Arabinose inoculated with pMG.002 at 10h30
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* 29C + shaking
  
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Result: no visible luminescence as of 19h30
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 +
==Start liquid cultures to test Nitrate reporter pMG.001==
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In LB + Cm
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{| class="wikitable"
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{| border="1"
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|-
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!  Nitrate [mM] !! LB !! 10% KNO3 [uL]
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|-
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| 0 || 5 || 0
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|-
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| 20 || 5 || 102
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|}
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[[Image:Nitrate_reporter_GFP_10-7-12.png]]
  
 
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Revision as of 08:59, 10 July 2012

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Luminescence test of pMG.002

  • Re-doing test for visible luminescence. NO ANTIBIOTIC ADDED
  • 20 mL LB + [100 uM, 1 mM] Arabinose inoculated with pMG.002 at 10h30
  • 29C + shaking

Result: no visible luminescence as of 19h30

Start liquid cultures to test Nitrate reporter pMG.001

In LB + Cm

Nitrate [mM] LB 10% KNO3 [uL]
0 5 0
20 5 102

Nitrate reporter GFP 10-7-12.png