IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/06: Difference between revisions
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== Biobricks checking== | == Biobricks checking== | ||
Analytical digests of pMG.001 (3 x colonies) and pMG.002 | |||
{| class="wikitable" | |||
{| border="1" | |||
|- | |||
! Reagent !! Volume | |||
|- | |||
| Plasmid DNA || 5 uL | |||
|- | |||
| Water || 12 uL | |||
|- | |||
| FastDigest Buffer || 2 uL | |||
|- | |||
| XbaI|| 0.5 uL | |||
|- | |||
| PstI|| 0.5 uL | |||
|} | |||
[[Image:KR003737.BMP]] | |||
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Revision as of 09:51, 6 July 2012
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assay Eglowli
--> we should have induced at 30°C or below. and look at the culture after 5 h of induction.
and same concentrations of Antibiotic and L arabinose
Biobricks checkingAnalytical digests of pMG.001 (3 x colonies) and pMG.002
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