Difference between revisions of "IGEM:Paris Bettencourt 2012/Notebooks/Delay group/day by day//2012/10/09"

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==Entry title==
 
 
Back from Amsterdam!
 
Back from Amsterdam!
  
 
We received the primers for the biobricking of the sRNA Delay system.
 
We received the primers for the biobricking of the sRNA Delay system.
*PCR
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==PCR==
 
We amplify the following products : sRNA (the sRNA generating part of the pBAD sRNA 7.9 plasmid), ColE2 (without RBS), aOmpF (stop before ATG) and YiaGp.  
 
We amplify the following products : sRNA (the sRNA generating part of the pBAD sRNA 7.9 plasmid), ColE2 (without RBS), aOmpF (stop before ATG) and YiaGp.  
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1° sRNA  amplified from strain #27. Expected product : 1746bp
 
1° sRNA  amplified from strain #27. Expected product : 1746bp
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2° ColE2  amplified from strain #5.  Expected product : 1801bp
 
2° ColE2  amplified from strain #5.  Expected product : 1801bp
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3° aOmpF amplified from strain #25    Expected product : 159bp
 
3° aOmpF amplified from strain #25    Expected product : 159bp
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4° YiaGp  amplified from MG1655    Expected product : 136bp
 
4° YiaGp  amplified from MG1655    Expected product : 136bp
  
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We run the PCR product on 2 gels : -1% agarose for 1°&2°
 
We run the PCR product on 2 gels : -1% agarose for 1°&2°
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-2% agarose for 3°&4°
 
-2% agarose for 3°&4°
  

Latest revision as of 22:06, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      

Back from Amsterdam!

We received the primers for the biobricking of the sRNA Delay system.

PCR

We amplify the following products : sRNA (the sRNA generating part of the pBAD sRNA 7.9 plasmid), ColE2 (without RBS), aOmpF (stop before ATG) and YiaGp.


1° sRNA amplified from strain #27. Expected product : 1746bp

2° ColE2 amplified from strain #5. Expected product : 1801bp

3° aOmpF amplified from strain #25 Expected product : 159bp

4° YiaGp amplified from MG1655 Expected product : 136bp

Tm : 52°C, elongation time = 1min, phusion.

We run the PCR product on 2 gels : -1% agarose for 1°&2°

-2% agarose for 3°&4°