IGEM:Paris Bettencourt 2012/Notebooks/Delay group/day by day//2012/10/09: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Back from Amsterdam! | Back from Amsterdam! | ||
We received the primers for the biobricking of the sRNA Delay system. | We received the primers for the biobricking of the sRNA Delay system. | ||
==PCR== | |||
We amplify the following products : sRNA (the sRNA generating part of the pBAD sRNA 7.9 plasmid), ColE2 (without RBS), aOmpF (stop before ATG) and YiaGp. | We amplify the following products : sRNA (the sRNA generating part of the pBAD sRNA 7.9 plasmid), ColE2 (without RBS), aOmpF (stop before ATG) and YiaGp. | ||
1° sRNA amplified from strain #27. Expected product : 1746bp | 1° sRNA amplified from strain #27. Expected product : 1746bp | ||
2° ColE2 amplified from strain #5. Expected product : 1801bp | 2° ColE2 amplified from strain #5. Expected product : 1801bp | ||
3° aOmpF amplified from strain #25 Expected product : 159bp | 3° aOmpF amplified from strain #25 Expected product : 159bp | ||
4° YiaGp amplified from MG1655 Expected product : 136bp | 4° YiaGp amplified from MG1655 Expected product : 136bp | ||
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We run the PCR product on 2 gels : -1% agarose for 1°&2° | We run the PCR product on 2 gels : -1% agarose for 1°&2° | ||
-2% agarose for 3°&4° | -2% agarose for 3°&4° | ||
Latest revision as of 22:06, 26 September 2017
Project name | Main project page Previous entry |
Back from Amsterdam! We received the primers for the biobricking of the sRNA Delay system. PCRWe amplify the following products : sRNA (the sRNA generating part of the pBAD sRNA 7.9 plasmid), ColE2 (without RBS), aOmpF (stop before ATG) and YiaGp.
2° ColE2 amplified from strain #5. Expected product : 1801bp 3° aOmpF amplified from strain #25 Expected product : 159bp 4° YiaGp amplified from MG1655 Expected product : 136bp Tm : 52°C, elongation time = 1min, phusion. We run the PCR product on 2 gels : -1% agarose for 1°&2° -2% agarose for 3°&4°
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