Difference between revisions of "IGEM:Paris Bettencourt 2012/Notebooks/Delay group/day by day//2012/10/09"

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==Entry title==
 
 
Back from Amsterdam!
 
Back from Amsterdam!
  
 
We received the primers for the biobricking of the sRNA Delay system.
 
We received the primers for the biobricking of the sRNA Delay system.
*PCR
+
==PCR==
 
We amplify the following products : sRNA (the sRNA generating part of the pBAD sRNA 7.9 plasmid), ColE2 (without RBS), aOmpF (stop before ATG) and YiaGp.  
 
We amplify the following products : sRNA (the sRNA generating part of the pBAD sRNA 7.9 plasmid), ColE2 (without RBS), aOmpF (stop before ATG) and YiaGp.  
 +
  
 
1° sRNA  amplified from strain #27. Expected product : 1746bp
 
1° sRNA  amplified from strain #27. Expected product : 1746bp
 +
 
2° ColE2  amplified from strain #5.  Expected product : 1801bp
 
2° ColE2  amplified from strain #5.  Expected product : 1801bp
 +
 
3° aOmpF amplified from strain #25    Expected product : 159bp
 
3° aOmpF amplified from strain #25    Expected product : 159bp
 +
 
4° YiaGp  amplified from MG1655    Expected product : 136bp
 
4° YiaGp  amplified from MG1655    Expected product : 136bp
  
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We run the PCR product on 2 gels : -1% agarose for 1°&2°
 
We run the PCR product on 2 gels : -1% agarose for 1°&2°
 +
 
-2% agarose for 3°&4°
 
-2% agarose for 3°&4°
  

Revision as of 04:09, 9 October 2012

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Back from Amsterdam!

We received the primers for the biobricking of the sRNA Delay system.

PCR

We amplify the following products : sRNA (the sRNA generating part of the pBAD sRNA 7.9 plasmid), ColE2 (without RBS), aOmpF (stop before ATG) and YiaGp.


1° sRNA amplified from strain #27. Expected product : 1746bp

2° ColE2 amplified from strain #5. Expected product : 1801bp

3° aOmpF amplified from strain #25 Expected product : 159bp

4° YiaGp amplified from MG1655 Expected product : 136bp

Tm : 52°C, elongation time = 1min, phusion.

We run the PCR product on 2 gels : -1% agarose for 1°&2°

-2% agarose for 3°&4°